YY1 anticorps

N° du produit ABIN7120908

Cet anticorps anti-YY1 est un anticorps Lapin Polyclonal détectant YY1 dans WB, ELISA et IHC. Adapté pour Humain, Souris, Rat, Poisson zèbre (Danio rerio), Porc, Singe, Lapin et Hamster.

Aperçu rapide pour YY1 anticorps (ABIN7120908)

Antigène: YY1 (YY1 Transcription Factor (YY1))

Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio), Porc, Singe, Lapin, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp YY1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-YY1 anticorps

Fonction: YY1 antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: YY1 transcription factor

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:2000, IHC: 1:50-1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur YY1

Antigène: YY1 (YY1 Transcription Factor (YY1))

Autre désignation: YY1

Sujet:

Synonyms: Transcriptional repressor protein YY1|Delta transcription factor|INO80 complex subunit S|NF-E1|Yin and yang 1 (YY-1)|YY1|INO80S

Background: Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3', some genes have been shown to contain a longer binding motif allowing enhanced binding, the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein(BMP)-mediated cardiac-specific gene expression(PubMed:15329343). Binds to SMAD binding elements(SBEs)(5'-GTCT/AGAC-3') within BMP response element(BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures(Holliday junctions). Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair, proposed to target the INO80 complex to YY1-responsive elements.

Poids moléculaire: 60 kDa, 75 kDa

ID gène: 7528

UniProt: P25490